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Journal of Aquatic Sciences 30 (2): 407-425 (2015)             DOI: http://dx.doi.org/10.4314/jas.v30i2.12 

 

         

 

SERUM BIOCHEMISTRY, BODY COMPOSITION AND BONE MINERALIZATION OF JUVENILE Clarias gariepinus FED PHYTASE-SUPPLEMENTED SOYABEAN-BASED DIETS

 

B.U. AKPOILIH

 

Department of Fisheries, University of Port Harcourt, Nigeria

 

Correspondence should be addressed to B.U. AKPOILIH: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

ABSTRACT

The effect of microbial phytase on phosphorus utilization by juvenile Clarias gariepinus was tested on two diets consisting of oil-extracted soyabean and full fat soyabean meal. Each of these diets was formulated to replace fish meal at 25%, 50%, 75%, and 100 % as basal controls (P0). Phytase was supplemented in another four groups of the diets at 250 FTU/g (P1), 500 FTU/g (P2), 750 FTU/g (P3) and 1000 FTU/g (P4), respectively. A total of 2132 fish (11.55±0.2g) were stocked in duplicate tanks (26 fish/tank) and fed the phytase diets at 3% body weight for 84 days. Result in trial 1 showed that whole body ash declined with increasing phytase compared to control (Tukey, P>0.05). Phytase supplementation increased serum phosphorus (250 FTU/g and 500 FTU/g). Total protein decreased with phytase (Tukey, P<0.05). Bone minerals declined significantly with phytase at all levels. In trial 2, there was no difference between phytase and control for serum parameters (Tukey, P>0.05). The highest ash and bone minerals were recorded for 250 FTU/g phytase (Tukey, P<0.05). In conclusion, the research showed 250 FTU/g improved phosphorus utilization in full fat soyabean, which may indicate higher phosphorus bioavailability compared to oil-extracted soyabean meal.

 

Key Words: Biochemistry, nutrition, plant protein, mineralization, Clarias gariepinus